CD44 Antikörper

Produktnummer ABIN118684

Dieser Maus Monoklonal Antikörper erkennt spezifisch CD44 in FACS, WB, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD44 Antikörper (ABIN118684)

Target: CD44

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD44 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: VFF-7

Produktdetails anti-CD44 Antikörper

Spezifität: SM1118P specifically recognises an epitope encoded by exon v6 on the variant portion of CD44.

Aufreinigung: Purified

Immunogen: Recombinant protein corresponding to exon variant 6 of the CD44 molecule. Spleen cells from immunised BALB/c micewere fused with cells of the P3X63Ag8.653 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: use 10 μL of 1:50 - 1:200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry: frozen sections 1:400-1:500, Paraffin sections: 1:200. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the product undiluted (in aliquots) at -20 °C.

Details zu CD44

Target: CD44

Andere Bezeichnung: CD44

Hintergrund: CD44 variant 6 is a cell surface glycoprotein expressed on lymphocytes, monocytes, and granulocytes. The CD44 Molecule belongs to a family of cellular adhesion molecule found on a wide range of normal and malignant cells in epithelial, mesothelial and haematopoietic tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been shown to be associated with metastasis and poor prognosis in certain human malignancies, such as breast cancer.Synonyms: CD44, CDw44, ECMR-III, Epican, Extracellular matrix receptor-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein I

Gen-ID: 960

NCBI Accession: NP_000601

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling